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Image Search Results
Journal: Oncology reports
Article Title: PLCε knockdown enhances the radiosensitivity of castration‑resistant prostate cancer via the AR/PARP1/DNA‑PKcs axis.
doi: 10.3892/or.2020.7520
Figure Lengend Snippet: Figure 3. Continued. PLCε regulates DNA damage repair by altering the AR/DNA‑PKcs pathway. (C) mRNA and (D) protein expression levels of PLCε, AR, DNA‑PKcs and H2AX in PCa cells. ‘#’ indicates irradiated cells. *P<0.05, **P<0.01 and ***P<0.001. PCa, prostate cancer; PLCε, phospholipase Cε; AR, androgen receptor.
Article Snippet: Following blocking with 5% skimmed milk for 2 h at room temperature, the membranes were incubated with primary antibodies targeting PLCε (1:500; cat. no. sc-28402; Santa Cruz Biotechnology, Inc.), AR (1:1,000; cat. no. 5153; CST Biological Reagents Co., Ltd.), PARP1 (1:1,000; cat. no. ab32138; Abcam), DNA-dependent protein kinase catalytic subunit (DNA-PKcs; 1:1,000; cat. no. ab168854; Abcam),
Techniques: Expressing, Irradiation
Journal: Oncology reports
Article Title: PLCε knockdown enhances the radiosensitivity of castration‑resistant prostate cancer via the AR/PARP1/DNA‑PKcs axis.
doi: 10.3892/or.2020.7520
Figure Lengend Snippet: Figure 7. PLCε knockdown inhibits the growth of CRPC in vivo. (A and B) Immunocompromised mice were subcutaneously injected with PLCε‑knockdown or control Bica‑R cells, and the tumor sizes and weights of the two groups are presented. (C) Comparison of positive rate of IHC in each group. (D) Expression of PLCε, AR, PARP1, DNA‑PKcs and γ‑H2AX in each group. Magnification, x200. *P<0.05 and ***P<0.001. PLCε, phospholipase Cε; CRPC, castration‑resistant prostate cancer; Bica‑R, Bicalutamide‑resistant; AR, Androgen receptor; PARP1, poly (ADP‑ribose) polymerase 1; H2AX, H2A histone family member X.
Article Snippet: Following blocking with 5% skimmed milk for 2 h at room temperature, the membranes were incubated with primary antibodies targeting PLCε (1:500; cat. no. sc-28402; Santa Cruz Biotechnology, Inc.), AR (1:1,000; cat. no. 5153; CST Biological Reagents Co., Ltd.), PARP1 (1:1,000; cat. no. ab32138; Abcam), DNA-dependent protein kinase catalytic subunit (DNA-PKcs; 1:1,000; cat. no. ab168854; Abcam),
Techniques: Knockdown, In Vivo, Injection, Control, Comparison, Expressing